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Image Search Results
Journal: Journal of Microscopy
Article Title: Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy
doi: 10.1111/jmi.12230
Figure Lengend Snippet: Combination and analysis of light and electron microscopy images recorded in the target region: A: 3D rendered fluorescence data set of the target cell. The fluorescence corresponds to the accumulation of internalized virus‐like particles. One layer of none confocal transmission contrast was included, to indicate the outline of the cell. B: Overlay of a single FIB‐SEM section with the corresponding section of the LSM image illustrates the correct guidance of FIB‐SEM imaging by functional fluorescence contrast. Reduced axial resolution of optical imaging results in a prolonged signal distribution along the z‐axis. Bar represents 1 μm. C: 3D FIB‐SEM dataset reveals the complete 3D structure of the virus‐like particles containing compartment (membrane is highlighted in green). D: Detailed view of the segmented compartment. The membrane is coloured in green and virus‐like particles are represented by red spheres.
Article Snippet: This process was performed in a semiautomated fashion using a custom built plug‐in for the
Techniques: Electron Microscopy, Fluorescence, Transmission Assay, Imaging, Functional Assay, Optical Imaging
Journal: Scientific Reports
Article Title: Global assessment of arteriolar, venular and capillary changes in normal tension glaucoma
doi: 10.1038/s41598-020-75784-1
Figure Lengend Snippet: Quantification of OCT-A Capillary Metrics in Normal and NTG eyes. Measurement of OCT-A capillary metrics are generated from ONH OCT-A images by a semi-automated customized MATLAB program (MATLAB R2017a, The MathWorks, Natick, MA, USA. https://www.mathworks.com/products/matlab.html ). (From left to right) ONH OCT-A images with the cpVD and cpFD measurement region defined. Binarization of the ONH OCT-A images with white pixels representing the blood vessels and black pixels representing the background (non-perfusion regions). Skeletonization of the ONH OCT-A images. Analysis and quantification of cpVD after removal of retinal arterioles and venules from the binarized ONH OCT-A images. Analysis and quantification of cpFD after removal of retinal arterioles and venules from the skeletonized ONH-OCT-A images. (Top: an example of normal eye. Bottom: an example of NTG eye).
Article Snippet: The OCT-A images were exported in grayscale from the built-in software and imported into a
Techniques: Generated